abstract：:The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were mad...

journal_title：Virus genes

authors： Dewey R,Semorille L,Crisci J,Grau O

更新日期：1996-01-01 00:00:00

abstract：:The search for retroviruses in human cancer cells has advanced with the discoveries of the retroviral reverse transcriptase, DNA hybridization and sequencing techniques, and polymerase chain reaction (PCR) technology. Each technological advancement has led to a better refinement and a better understanding of latent en...

journal_title：Virus genes

authors： Becker Y

更新日期：1995-02-01 00:00:00

abstract：:Two or three regions containing three or more successive newly defined heptads of a-d hydrophobic amino acid repeats have been located in the cDNA-derived amino acid sequences of glycoprotein G of all rhabdoviruses examined (rabies, vesicular stomatitis, fish, and plant rhabdoviruses) by computer search. These new hep...

journal_title：Virus genes

authors： Coll JM

更新日期：1995-01-01 00:00:00

abstract：:Cytotoxic T cells that recognize dengue fever viral (DFV) peptides were reported. To predict the cleavage pattern of DFV proteins by cytoplasmic proteasomes into nonapeptides with motifs fitting known HLA class I molecules, the computer program "Findpatterns" was used. In this study the combined amino acid motifs for ...

journal_title：Virus genes

authors： Becker Y

更新日期：1995-01-01 00:00:00

abstract：:Human spuma retrovirus (HSRV) belongs to retroviruses that possess a complex genome organization. HSRV carries at least three extra genes in the region between env and the 3' long terminal repeat, which are not found in simple retroviruses. Via alternative splicing, these HSRV genes can encode several proteins. To gen...

journal_title：Virus genes

authors： Adachi A,Sakai H,Tokunaga K,Kawamura M

更新日期：1995-01-01 00:00:00

abstract：:Two ribozyme genes were designed to cut within the VP4 and VP3 sequences of foot and mouth disease virus (FMDV) Asia1 serotype genome. The two genes were synthesized and cloned into pBluescript under the control of the T3 promoter. The ribozyme designed to cut the VP4 gene contained two catalytic sequences targeted to...

journal_title：Virus genes

authors： Stram Y,Molad T

更新日期：1995-01-01 00:00:00

abstract：:Comparison of the genomic organization of variola and vaccinia viruses has been carried out. Molecular factors of virulence of these viruses is the focus of this review. Possible roles of the genes of soluble cytokine receptors, complement control proteins, factors of virus replication, and dissemination in vivo for v...

journal_title：Virus genes

authors： Shchelkunov SN

更新日期：1995-01-01 00:00:00

abstract：:Viroids, the smallest and simplest agents of infectious disease, cause a number of economically important diseases of crop plants. Present evidence indicates that most of these diseases originated recently (in the 20th century) by chance transfer of viroids from endemically infected wild plants or by use of viroid-inf...

journal_title：Virus genes

authors： Diener TO

更新日期：1995-01-01 00:00:00

abstract：:Historically, viral evolution has often been considered from the perspective of the ability of the virus to maintain viral pathogenic fitness by causing disease. A predator-prey model has been successfully applied to explain genetically variable quasi-species of viruses, such as influenza virus and human immunodeficie...

journal_title：Virus genes

authors： Shadan FF,Villarreal LP

更新日期：1995-01-01 00:00:00

abstract：:Recent studies indicate that alphaherpesviruses express latency associated transcripts (LATs) from the antisense strand of immediate early (IE) genes of the viral genome. It has been discussed that LATs containing extended open reading frames (ORFs) might be translated into protein products. We found that a salient fe...

journal_title：Virus genes

authors： Boldogköi Z,Murvai J

更新日期：1994-09-01 00:00:00

abstract：:Caliciviruses are nonenveloped with a polyadenylated genome of approximately 7.6 kb and a single capsid protein. The "RNA Fold" computer program was used to analyze 3'-terminal noncoding sequences of five feline calicivirus (FCV), rabbit hemorrhagic disease virus (RHDV), and two San Miguel sea lion virus (SMSV) isolat...

journal_title：Virus genes

authors： Seal BS,Neill JD,Ridpath JF

更新日期：1994-07-01 00:00:00

abstract：:By using a phage vector (lambda ZAP II) and the mRNA extracted from IMR-32 cells infected with the RC.HL strain of rabies virus, we constructed a cDNA library from which four nucleoprotein (N)-specific cDNA clones were obtained by Southern blot hybridization. These clones contained a cDNA insert of about 1.4 kb, in wh...

journal_title：Virus genes

authors： Goto H,Minamoto N,Ito H,Sugiyama M,Kinjo T,Mannen K,Mifune K,Kawai A

更新日期：1994-03-01 00:00:00

abstract：:Herpesvirus saimiri induces acute lymphomas and leukemias in primates and rabbits. Sequence divergence of the right end unique region of the genome classifies virus strains into three groups (A, B, and C), and previous studies have demonstrated correlation between DNA grouping and oncogenicity. In order to relate diff...

journal_title：Virus genes

authors： Geck P,Whitaker SA,Medveczky MM,Last TJ,Medveczky PG

更新日期：1994-01-01 00:00:00

abstract：:Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one la...

journal_title：Virus genes

authors： Zhang S,Jones MC,Barker P,Davies JW,Hull R

更新日期：1993-06-01 00:00:00

abstract：:Statistically significant sequence similarity was revealed between the gene A32 product of vaccinia virus (VV), gene I products (gpI) of filamentous single-stranded DNA bacteriophages, and IVa2 gene products of adenoviruses. Four conserved sequence motifs were delineated, the two N-proximal of which correspond to the ...

journal_title：Virus genes

authors： Koonin EV,Senkevich TG,Chernos VI

更新日期：1993-02-01 00:00:00

abstract：:We have described three mutant strains of Pseudorabies virus that contain mutations in the signal sequence coding region of a nonessential envelope glycoprotein, gIII. The alterations disrupt, truncate, or eliminate the hydrophobic core domain of the signal sequence. Each mutant was assayed for its ability to promote ...

journal_title：Virus genes

authors： Ryan P,Robbins A,Whealy M,Enquist LW

更新日期：1993-02-01 00:00:00

abstract：:The nucleotide sequence of the genome segment (S6) encoding the nonstructural protein NS1 of an Australian isolate of bluetongue virus serotype 20 (BTV 20) has been determined from a series of overlapping cDNA clones synthesized using two terminal 15-mer oligonucleotides as primers. The gene consists of 1769 nucleotid...

journal_title：Virus genes

authors： Cowley JA

更新日期：1992-11-01 00:00:00

abstract：:The major immunogenic protein VP2 from a pathogenic field isolate (variant A virus) of infectious bursal disease virus (IBDV) was cloned and sequenced to examine antigenic variations. The VP2 open reading frame consists of 1509 nucleotides and codes for a 503 amino acid protein. Overall, the VP2 amino acid sequence of...

journal_title：Virus genes

authors： Lana DP,Beisel CE,Silva RF

更新日期：1992-08-01 00:00:00

abstract：:Bacteriophage T7 RNA polymerase and a derivative containing a nuclear localization signal were transiently expressed in CV-1 cells and were shown to localize to the cytoplasm and nucleus, respectively. A vector was constructed containing T7 promoter and transcription terminator sequences flanking a picornaviral 5' unt...

journal_title：Virus genes

authors： Wilk T,Mierswa H,Kräusslich HG,Dunn JJ,Bosch V

更新日期：1992-08-01 00:00:00

abstract：:Cauliflower mosaic virus (CaMV) gene I encodes a protein (P1) that has been implicated in the control of virus movement in infected plants. To assist in the characterization of the mechanism of action of P1, gene I has been expressed efficiently in Spodoptera frugiperda (Sf) cells using recombinant baculovirus. Contro...

journal_title：Virus genes

authors： Maule AJ,Usmany M,Wilson IG,Boudazin G,Vlak JM

更新日期：1992-01-01 00:00:00

abstract：:Herpesvirus of turkeys (HVT) vaccine is used worldwide to immunize chickens against Marek's disease (MD). Polyclonal antiserum directed against one virus cross-reacts with proteins of the other, while only 5% homology at the DNA level was demonstrated between the two viruses. A partial library of HVT DNA fragments ran...

journal_title：Virus genes

authors： Levy H,Maray T,Davidson I,Malkinson M,Becker Y

更新日期：1991-07-01 00:00:00

abstract：:The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV). The HBV-DNA of subtype adr derived from a hep...

journal_title：Virus genes

authors： Klein R,Schröder CH,Zentgraf H

更新日期：1991-04-01 00:00:00

abstract：:Endogenous retrovirus-like sequences that are homologous to the multigenic murine and Syrian hamster intracisternal type A particle (IAP) genes can be detected in very few copies in the Chinese hamster (CH) genome. They were cloned from a CHO gene library and two recombinants, harboring the major IAP-like DNA genes ch...

journal_title：Virus genes

authors： Servenay M,Kupiec JJ,Peries J,Emanoil-Ravier R

更新日期：1990-12-01 00:00:00

abstract：:Complementary DNAs (cDNAs) to the RNA genome of a necrotic strain of potato virus Y in Japan (Hokkaido Univ. isolate of PVY-T:PVY-TH) were synthesized and cloned into a plasmid pBR322. About 4.3 kbp of the cDNA sequence containing the 3'-poly(A) tract of PVY-TH was inserted into a recombinant plasmid pBRYT88. The coat...

journal_title：Virus genes

authors： Hataya T,Sano T,Ohshima K,Shikata E

更新日期：1990-12-01 00:00:00

abstract：:The genomes (188 kbp) of the prototype Molluscum contagiosum virus type 1 (MCV-1) and a variant strain (MCV-1v) were characterized by construction of the physical maps of the viral DNA for the restriction enzymes BamHI, ClaI, EcoRI, and HindIII using a defined gene library harboring the DNA sequences of the MCV-1 geno...

journal_title：Virus genes

authors： Bugert J,Rösen-Wolff A,Darai G

更新日期：1989-11-01 00:00:00

abstract：:DNA fragments containing the secretory glycoprotein A (gA) gene of Marek's disease virus type 1 (MDV1) were cloned from the DNA libraries of very virulent Md5 and virulent BC-1 strains and sequenced. Two open reading frames (ORF1 and ORF2) were identified for both strains. The ORF1 has the potential to code for a prot...

journal_title：Virus genes

authors： Ihara T,Kato A,Ueda S,Ishihama A,Hirai K

更新日期：1989-11-01 00:00:00

abstract：:Varicella-zoster virus (VZV) DNA was detectable by in-situ hybridization in blood mononuclear cells (MNCs) of patients with varicella or zoster for 2-56 days after the onset of a rash. VZV DNA was present in many MNCs from one acute varicella patient 2 days after the onset of the rash and was rarely found in MNCs duri...

journal_title：Virus genes

authors： Gilden DH,Devlin M,Wellish M,Mahalingham R,Huff C,Hayward A,Vafai A

更新日期：1989-08-01 00:00:00

abstract：:Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5 X 10(6) daltons (R1) and 0.76 X 10(6) daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro tra...

journal_title：Virus genes

authors： Hari V,Gadh IP,Baunoch D,Das P

更新日期：1989-05-01 00:00:00

abstract：:A BK virus isolate cloned from the urine of an immunosuppressed patient was identified as the WW strain. Fine mapping using four base-pair restriction enzymes identified a number of small deletions and insertions relative to prototype BK. The finding of a transcriptional control region with a structure similar to that...

journal_title：Virus genes

authors： Rubinstein R,Harley EH

更新日期：1989-03-01 00:00:00

abstract：:The nucleotide sequence of the 3' terminal region of potato virus Y (PVY) was determined. Starting with a poly(A) tail of 18 residues a non-coding region of 335 nucleotides precedes the region encoding for the virus coat protein (cp) 801 nucleotides long ending with a TGA. This region was located by comparing the pred...

journal_title：Virus genes

authors： Rosner A,Raccah B

更新日期：1988-06-01 00:00:00

abstract：:We have extended our studies using the mouse lymphoid cell system to determine if polyomavirus (Py) cell type specificity is a consequence of the simple presence of either the A and B enhancers, or if specificity results from specific combinations or arrangements of the individual enhancer elements. Mouse fibroblasts ...

journal_title：Virus genes

authors： Campbell BA,Villarreal LP

更新日期：1987-11-01 00:00:00

abstract：:Six recombinants of New York Board of Health (NYBH) vaccinia virus containing cDNA for Challenge Virus Standard (CVS) rabiesvirus glycoprotein (G) were produced by directing gene insertion into the vaccinia thymidine kinase (TK) locus. To regulate expression of G the promoter P7.5 (functions at early and late times po...

journal_title：Virus genes

authors： Esposito J,Brechling K,Baer G,Moss B

更新日期：1987-11-01 00:00:00